Stem Cell

Stem Cell Analysis Navigator

© 2025 S. Tritley, C. Rouse, E. Brey, A. Qutub

FIJI (ImageJ)
NIH / community (1997)

Platform: Python, Java, etc

Summary: Open-source software workbench with BigDataViewer/BigStitcher for Lightsheet, Sci-Java plugin ecosystem, etc.

Website
Citation
CellProfiler
Broad Institute (2005)

Platform: Python, Plugins

Summary: Modular pipelines (v. 4) with object analysis and classification (Cell Painting, etc) features.

Website
Citation
NeuroMetrics
Academic (2007)

Platform: ImageJ

Summary: Semi-automated processing of large sets of neuronal images.

Website
Citation
NeuriteTracer
Academic (2008)

Platform: ImageJ

Summary: Automated tracing of neurites from fluorescence images.

Website
Citation
CATMAID
HHMI Janelia project (2009)

Platform: Web

Summary: Collaborative annotation of large EM volumes. Remote tracing, connectomics queries.

Website
Citation
Ilastik
Heidelberg University (2011)

Platform: Python

Summary: Interactive learning and segmentation toolkits for complex datasets.

Website
Citation
NeuriteQuant
QBI (open-source imagej) (2011)

Platform: Fiji macro/plugin

Summary: High-throughput rapid neurite outgrowth quantification from 2D images.

Website
Citation
NeurphologyJ
ImageJ plugin (2011)

Platform: ImageJ

Summary: ImageJ plugin for neurite outgrowth quantification.

Website
Citation
NeuronJ
Academic (2012)

Platform: ImageJ

Summary: ImageJ plugin for neurite outgrowth quantification. 10.1007/ s12021-011-9134-2

Website
Citation
Knossos
Universities of Jyväskylä and Zurich (2012)

Platform: Unknown

Summary: Single cell models to map single cell models to map single neuron traces and stitch volume electron microscopy

Website
Citation
3D-Cell-Annotator
Institut Pasteur (2012)

Platform: C++, Python

Summary: Software for tracking cells and analyzing 3-dimensional microscopy images

Website
Citation
Neuron-Spine-Analyzer
Academic (2013)

Platform: ImageJ

Summary: Open-source ImageJ plugin for analysis of spine morphology

Website
Citation
NEMO
Academic (2013)

Platform: MATLAB

Summary: Neurite outgrowths in spinal cord neuronal root ganglia

Website
Citation
neurite-mosaic
Academic (2013)

Platform: ImageJ

Summary: Batch processing of landscape images for neuronal morphology.

Website
Citation
Neurolucida 360
MBF Bioscience (2014)

Platform: MATLAB

Summary: Traces neurites and cell bodies, quantifies neurite-neurite populations

Website
Citation
MorphoNeuroN
Massana Pons et al. (2014)

Platform: ImageJ

Summary: Quantifies morphology of neurons and density of neurite networks.

Website
Citation
Neuronstudio J 360
MBF Bioscience (2015)

Platform: MATLAB

Summary: 3D neuron reconstruction, dendritic spine analysis.

Website
Citation
Neuron Image Analyzer
Academic (2015)

Platform: Windows

Summary: Extracts neuronal data from low-quality images using LoG and graphical models.

Website
Citation
vaa3d
Janelia / community (2016)

Platform: C++/Qt

Summary: Open-source SIM-based software for tracing with parallel (CPU) trees and plugins.

Website
Citation
NeuronJ 2
Academic (2016)

Platform: Windows

Summary: Individualized tracing/tracking of neurons.

Website
Citation
NeuroAnatomy Decomposer
Academic (2016)

Platform: MATLAB

Summary: Decomposes neuronal arbors into dendrites from morphological Micro-CT volumes.

Website
Citation
DeepCell
Van Valen Lab (Caltech) (2016)

Platform: Web - Python

Summary: Deep learning library for single-cell analysis from images.

Website
Citation
QPath
QPath cousins (2017)

Platform: Java, Scripting, Plugins

Summary: WSi module with batch processing, integrates StarDist, WSi deconvolution.

Website
Citation
GAIN
Academic (2017)

Platform: ImageJ

Summary: Resolves cell-by-cell morphology, individual neurites, etc.

Website
Citation
Vaa3d-Neurolucida
Hanchuan Peng et al. (2018)

Platform: C++, Cross-platform

Summary: 3D reconstruction, tracing, cell-pop visualization, connectomics analysis.

Website
Citation
NeuroMorph
Adam Sefelin et al. (EPFL) (2018)

Platform: Python

Summary: Morphometric analysis and visualization of 3D-reconstructed neurons.

Website
Citation
ChronoWizard
Academic (2018)

Platform: Python (Blender addon)

Summary: Software for m-Rotundus H51 image analysis.

Website
Citation
Simple Neurite Tracer
Academic (2018)

Platform: ImageJ

Summary: 3D semi-automated tracing and reconstruction, includes Sholl analysis, etc.

Website
Citation
Neural-fresco
Academic (2018)

Platform: Python

Summary: Deep learning tools for neuron tracing.

Website
Citation
Synergy
Thiels Lab (2018)

Platform: Unknown

Summary: Extracts synaptic vesicles from EM images.

Website
Citation
SpatialDE
Svensson et al. (2018)

Platform: Python

Summary: Method for analyzing single-cell gene expression data.

Website
Citation
StarDist
CSBD (2018)

Platform: Python

Summary: Task for analyzing single-cell gene expression data.

Website
Citation
Traction Pipeline
Mosconi Bio (2018)

Platform: MATLAB

Summary: UL model for star-convex objects/nuclei segmentation with D-H capacity, Python.

Website
Citation
Cellpose (Cellpose/DeepVess)
CSHL (2019)

Platform: Command line

Summary: Analysis pipeline for single-cell DNA sequencing data.

Website
Citation
ST Viewer
SciLifeLab (2019)

Platform: Fiji, Python

Summary: Set of tools (ST viewer, UMAP) for analysis of spatial transcriptomics data.

Website
Citation
Giotto DSP
NanoString Technologies (2019)

Platform: Web-based

Summary: Visualization tool for GeoMx digital spatial profiler data readouts.

Website
Citation
Pegasus
Targeted collaboration (2019)

Platform: Promoters

Summary: Python tool for GeoMx digital spatial profiler data.

Website
Citation
Seurat
Academic (2019)

Platform: Python, TensorFlow

Summary: Fast and accurate deconvolution of spatial transcriptomics data.

Website
Citation
Squidpy
Helmholtz Munich (2019)

Platform: Python, R

Summary: Efficient and scalable analysis of spatial molecular data.

Website
Citation
Space Ranger
10x Genomics (2019)

Platform: C++, Go, Rust, Python, R

Summary: Analysis pipeline for Visium Spatial Gene Expression data.

Website
Citation
MOSAI
Project MONAI (i.e. NVIDIA) (2019)

Platform: Python, Pytorch

Summary: Domain-optimized and training/inference tasks for biomedical imaging.

Website
Citation
Deep-n-REACH
Academic (2019)

Platform: Python, Deep learning based

Summary: Toolbox for brightfield/fluorescence images with custom workflows.

Website
Citation
Synapse-Count-Tool
ImageJ macro (2020)

Platform: Python

Summary: Measures and classifies neurites, detects variations in neurite growth.

Website
Citation
AxonDeepSeg
NeuroPoly lab at Polytechnique Montreal (2020)

Platform: Pytorch

Summary: Performs cell-in-cell models, 2D/3D segmentation, slicing, export and contributor diagnostics.

Website
Citation
Cellpose (incl. Neurite Tracer)
Pachitariu Lab & collaborators (2020)

Platform: Python GUI, Jupyter

Summary: Semi-automated 2D/3D tracing, export SWC/SVG files, morphometrics and Sholl analysis.

Website
Citation
ShapoShape
Fiji plugin (2020)

Platform: MATLAB, R

Summary: Pipeline for 3D cell segmentation and analysis.

Website
Citation
BayerSpace
Zhao et al. (2021)

Platform: R

Summary: Reversible method for spatial gene expression analysis.

Website
Citation
Explainable-cell-analysis
Academic (2021)

Platform: Python

Summary: Weighted graph from wavelet-tree segmentation. Cell classification via SVM and Grad-CAM.

Website
Citation
NeurTEx
Academic (2021)

Platform: Deep learning platform

Summary: Segments brightfield images, tracks neurites, evaluates morphology.

Website
Citation
DeepHisto
EPFL/LCSB (2022)

Platform: Fiji plugin + web-based

Summary: End-to-end and unified analysis tool, neighborhood graphs, interconnects with Giotto Invest.

Website
Citation
Giotto
Dries et al. (2021)

Platform: R

Summary: End-to-end and unified analysis tool, neighborhood graphs, interconnects with Giotto Invest.

Website
Citation
LightMyCells
Florian-Reyes et al (2021)

Platform: Web app (Python)

Summary: Method for discovering spatial transcriptomics data

Website
Citation
SPOTlight
Enriquez-Lab (2021)

Platform: Python + R

Summary: Collab-enabled tool to triangulate 2L models (scanpy, Giotto, Seurat) for integration with single-cell data.

Website
Citation
Aivia
DRVision (commercial) (2022)

Platform: Windows

Summary: UL modeling, image classification and segmentation, integration with Caffe.

Website
Citation
Cytofast
Academic (2022)

Platform: Web + Python

Summary: Builds spatial/partitioned graphs from matrices or radiance for large-scale network profiling.

Website
Citation
Quanti.us
Academic (2022)

Platform: Python

Summary: M-FISH image analysis framework.

Website
Citation
Squidpy
Theis lab / open-source (2022)

Platform: Python + R

Summary: Unifies data and molecular profiles, neighborhood graphs, preparation for downstream analyses.

Website
Citation
Xenium Onboard Analysis
10x Genomics (2022)

Platform: Command line

Summary: Analysis pipeline for Xenium in-situ data.

Website
Citation
Imaris 10
Oxford Instruments (commercial) (2023)

Platform: Windows

Summary: Filament Tracer for organoids/neurons, batch analysis, vesicle/organelle/object tracking, object-object / object-group/ i-graphlet analysis.

Website
Citation
Seurat 5
Academic (2023)

Platform: R

Summary: Seurat v.5 integrates RNA-seq with spatial transcriptomics data.

Website
Citation
Vitessce
Harvard Medical School (2024)

Platform: Web-based, React, Python,

Summary: Visualization tool for multi-omics single-cell data.

Website
Citation
scanpyCellCall
Bader Lab, Network Biology Collaborative Centre (2024)

Platform: Python

Summary: Integrated platform for single-cell and spatial omics analysis.

Website
Citation
HTP2S
Cancer Institute of New South Wales (2024)

Platform: Python

Summary: High-throughput image-processing software for quantifying DNA damage and nuclear size.

Website
Citation
NeuroQuant-fy
Stanley Center (Broad) (2024)

Platform: Python

Summary: Automatic detection of neural boundaries, calculating neurite length and number.

Website
Citation
NeuroAnatomy Tracer
Utrecht University (commercial) (2024)

Platform: Open-source (Python-based)

Summary: Semi-automatic pipeline for processing up to 10-Terabyte images for 3D reconstruction and tracing, Sholl and connectome analysis, cell and sub-cellular distribution.

Website
Citation
STAToCet
Ultivue (2024)

Platform: Cloud-based

Summary: AI platform for multiplex immunofluorescence data analysis, including cell segmentation, phenotyping, and high-throughput processing.

Website
Citation
SyNapse
Academic (2024)

Platform: ImageJ-based

Summary: Automated pipeline for detecting synapses from immunofluorescence images, using Stardist and SynQuant for thresholding and puncta.

Website
Citation
Space Ranger Multi
10x Genomics (2024)

Platform: Binary (pipeline)

Summary: Analysis pipeline for Visium Spatial Gene Expression data. Integrates imaging data from CytAssist, provides automatic image processing and control and performs segmentation.

Website
Citation
Vispro
Academic (2024)

Platform: Python, Blender, Unity

Summary: Automated image processing for volumes, including cortical thickness, tissue segmentation, and neuronal tracing/rendering.

Website
Citation
cytoNet
Academic (2022)

Platform: Web, Python, MATLAB

Summary: Builds spatial/functional graphs from masks or calcium time series; network metrics.

Website
Citation
avaris Cloud
Zeiss (2018)

Platform: Web, Python, MATLAB

Summary: Cloud-based platform for 2D/3D image analysis with community/own modules, trainable DL models.

Website
Citation